Protein labeling … on the go

In protein bioconjugation, isolation of homogeneously labeled proteins has been a challenging task. A recent paper (Chem. Sci. 2015) by Francis et al outlines a smart approach where the label includes an affinity tag allowing separation of monolabeled protein from bis- or tris-labeled conjugates. The method can potentially draw comparison with combinatorial chemistry for generation of library of variably labeled proteins.

Figure. Image derived from Chem. Sci. 2015, 6, 2596-2601